汉滩病毒感染EA.hy926细胞上调固有免疫相关分子表达的研究

王晓艳; 李　璟; 申焕君; 杜　虹; 权会琴; 李光玉; 王平忠

文章摘要

汉滩病毒感染EA.hy926细胞上调固有免疫相关分子表达的研究

Study on expression of innate immunity-related molecules in EA. hy926 cells infected by hantaan virus

DOI：10.3969/j.issn.1007-8134.2020.05.004

中文关键词: 汉滩病毒 EA.hy926细胞固有免疫

英文关键词: hantaan virus EA.hy926 cell innate immunity

基金项目:国家自然科学基金面上项目（81373118）；陕西省自然科学基金一般项目（2020SF-102）

作者	单位
王晓艳	空军军医大学第二附属医院传染科
李　璟	空军军医大学第二附属医院传染科
申焕君	空军军医大学第二附属医院传染科
杜　虹	空军军医大学第二附属医院传染科
权会琴	空军军医大学第二附属医院传染科
李光玉	Galveston, Department of Pathology, The University of Texas Medical Branch, America
王平忠	空军军医大学第二附属医院传染科

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中文摘要:

目的　探讨汉滩病毒（hantaan virus, HTNV）感染EA.hy926细胞诱导抗病毒固有免疫相关分子的表达，为建立HTNV感染的细胞模型提供依据。方法　将HTNV感染EA.hy926细胞，利用间接免疫荧光和实时荧光定量PCR技术于不同感染时间段，检测EA.hy926细胞中模式识别受体、细胞因子及抗病毒相关分子的mRNA表达水平。结果　HTNV感染EA.hy926细胞后，随着感染时间的持续，核蛋白mRNA相对表达倍数显著上调，且不同感染时间段差异具有统计学意义（P＜0.05）；与未处理组相比，HTNV感染EA.hy926后，Toll样受体3、RIG-I和MDA5模式识别受体mRNA相对表达倍数均显著上调（P均＜0.05），IL-6、IL-10、IFN-β和CCL5细胞因子mRNA相对表达倍数均显著上调（P均＜0.05），ISG15、MxA、OAS1、IFITM1和IFITM3抗病毒相关分子mRNA相对表达倍数均显著上调（P均＜0.05）。结论　HTNV感染EA.hy926细胞后，可上调抗病毒固有免疫相关分子的表达，该细胞可以作为体外HTNV感染的细胞模型。

英文摘要:

Objective　To investigate the expression of antiviral innate immunity-related molecules in EA.hy926 cells infected by hantaan virus (HTNV), and to provide the basis for establishing the cell model of HTNV infection. Methods　EA.hy926 cells were infected with HTNV, and mRNA expression levels of pattern recognition receptors, cytokines and antiviral molecules in EA.hy926 cells were detected using indirect immunofluorescence and real-time fluorescent quantitative polymerase chain reaction at different infection periods. Results　After EA.hy926 cells were infected with HTNV, with the duration of infection, the relative expression multiples of nucleocapsid protein mRNA were significantly upregulated, and there were statistically significant differences among different periods of infection (P＜0.05). Compared with untreated group, the mRNA relative expression multiples of Toll-like receptor 3, RIG-I and MDA5 pattern recognition receptors were significantly upregulated (P＜0.05), the mRNA relative expression multiples of IL-6, IL-10, IFN-β and CCL5 were significantly upregulated (P＜0.05), and the mRNA relative expression multiples of antiviral molecules such as ISG15, MxA, OAS1, IFITM1 and IFITM3 were significantly upregulated in the HTNV-infected EA.hy926 cells (P＜0.05). Conclusions　HTNV can upregulate the expression of antiviral innate immunity-related molecules in EA.hy926 cells, and EA.hy926 cells can be used as a cell model for HTNV infection in vitro.

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