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| HIV感染者滤泡细胞毒性T细胞与疾病进展的关系及功能分析 |
| Correlation of TFC with disease progression and its function in HIV patients |
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| DOI：10.3969/j.issn.1007-8134.2020.06.004 |
| 中文关键词: TFC HIV PD-1 杀伤功能 分泌功能 |
| 英文关键词: TFC HIV PD-1 killing function secretory function |
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| 中文摘要: |
| 目的 探索HIV感染者滤泡细胞毒性T细胞（follicular cytotoxic T cells, TFC）（即CXCR5+CD8+ T细胞）与疾病进展的关系，并将其与CXCR5-CD8+ T细胞对比分析。方法 入组HIV感染未治疗患者，抗病毒治疗后免疫重建成功和免疫重建失败患者，收集并分离其外周血单个核细胞，经流式染色及体外培养后分析其数量、表型及功能。结果 HIV感染者TFC的数量明显增加，TFC与疾病进展呈负相关。与CXCR5-CD8+ T细胞相比，TFC上PD-1的水平明显增加，TFC的分泌功能较强而杀伤功能较弱。结论 在HIV感染中，TFC与疾病进展呈负相关。TFC与CXCR5-CD8+ T细胞在PD-1的表达和功能上均有一定的差异。 |
| 英文摘要: |
| Objective To investigate the relationship between follicular cytotoxic T cells (TFC) and disease progression in HIV patients and analyze the difference between TFC and CXCR5-CD8+ T cells. Methods Peripheral blood mononuclear cells were collected and isolated from untreated HIV patients, and the patients with successful and failed immune reconstitution after antiviral therapy. The quantity, phenotype and function of these cells were analyzed using flow cytometry staining and in vitro culture. Results The number of TFC increased significantly in HIV infected patients. TFC was negatively correlated with the progression of HIV. Compared with CXCR5-CD8+ T cells, the level of PD-1 on TFC was significantly increased, and the secretion function of TFC was stronger while the killing function was weaker than that of CXCR5-CD8+ T cells. Conclusions TFC is negatively correlated with disease progression during HIV infection. PD-1 expression and function of TFC are very different from those of CXCR5-CD8+ T cells. |
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