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| 基于生物信息学方法分析雌激素受体相关基因在女性结核病患者中的表达及意义 |
| Expression and significance of estrogen receptor-associated genes in females with tuberculosis based on bioinformatics technical analysis |
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| DOI：10.3969/j.issn.1007-8134.2020.06.012 |
| 中文关键词: 结核 女性 生物信息学分析 |
| 英文关键词: tuberculosis female bioinformatics analysis |
| 基金项目:国家自然科学基金青年科学基金（81600388） |
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| 中文摘要: |
| 目的 探索女性结核病患者雌激素受体相关联的基因并进行实验验证。方法 下载GSE114911、GSE54992、GSE83456 3个数据集的基因表达谱。使用R软件探索来自女性的结核组织样本与正常组织样本之间的差异基因。在线数据库DAVID用于差异基因的富集分析。使用相互作用基因/蛋白质检索的搜索工具和Cytoscape软件对雌激素受体基因和差异基因进行网络构建。取我院女性肺结核患者肺结核组织和正常肺组织标本，运用实时定量PCR、Western blot检测关键基因在肺结核组织和正常肺组织中的表达水平。结果 3个数据集的交集显示了24个差异基因。使用GO功能注释将24个差异基因分为生物学过程、分子功能和细胞组分3个类别。随后进行了KEGG分析，总共有10个差异基因和雌激素受体密切相关。其中，CCL20、CXCR3与雌激素受体关联最为密切的基因。运用实时定量PCR发现，肺结核组织样本中的CCL20 mRNA表达水平为2.56±0.34，低于配对的正常肺组织的7.88±0.51（t=-2.375，P=0.003），CXCR3 mRNA表达水平为8.35±1.76，高于配对的正常肺组织的1.24±0.34（t=1.384，P=0.008）；Western blot验证结果与实时定量PCR结果趋势一致。结论 本研究通过多个数据库对CCL20、CXCR3进行生物信息学分析，发现雌激素受体相关基因可能在女性结核病中发挥作用，为结核病的分子机制提供新的见解。 |
| 英文摘要: |
| Objective To explore the genes associated with estrogen receptor in tuberculosis female patients and to perform an experimental verification. Methods The gene expression profiling of GSE114911, GSE54992, GSE83456 data sets were downloaded. R software was used to investigate the differentially expressed genes (DEGs) between tuberculosis tissue samples and normal tissue samples from female patients. The online database for DAVID was used to analyze the enrichment of DEGs. Using the search tool of interaction gene/protein retrieval and Cytoscape software, the network between estrogen receptor gene and DEGs was constructed. Real-time quantitative PCR and Western blot assay were used to detect the expression level of key genes in pulmonary tuberculosis tissues and normal lung tissues of female tuberculosis patients in our hospital. Results The intersection of 3 data sets showed 24 DEGs. Using the functional annotation of GO, 24 DEGs were divided into 3 categories: biological process, molecular function and cellular component. The KEGG analysis was subsequently carried out. A total of 10 DEGs were closely associated with estrogen receptor, in which CCL20 and CXCR3 had the closest association with estrogen receptor. Real-time quantitative PCR showed that the expression level of CCL20 mRNA in pulmonary tuberculosis tissue 2.56±0.34 was lower than that in matched normal lung tissue 7.88±0.51 (t=-2.375, P=0.003), and the expression level of CXCR3 mRNA in pulmonary tuberculosis tissue 8.35±1.76 was higher than that in matched normal lung tissue 1.24±0.34 (t=1.384, P=0.008). The results of Western blot assay were consistent with the results of real-time quantitative PCR. Conclusions In this study, the bioinformatics analysis of CCL20 and CXCR3 conducted through several databases has demonstrated that estrogen receptor-associated genes may play a role in female tuberculosis and provide new insights into the molecular mechanism of tuberculosis. |
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