文章摘要
黄芪多糖对肝癌Hep G2.215 细胞的抑制作用及其机制研究
Study on the Inhibitory effect of Astragalus polysaccharides on Hep G2.215 cells and its mechanism
  
DOI:10.3969/j.issn.1007-8134.2022.02.006
中文关键词: 黄芪多糖  Hep G2.215  CCK-8  细胞克隆  细胞周期  细胞凋亡  肝癌  HBV
英文关键词: Astragalus polysaccharide  Hep G2.215  CCK-8  cell cloning  cell cycle  apoptosis  hepatocellular carcinoma  HBV
基金项目:国家自然科学基金(81603552);北京市自然科学基金(7212172);广西自然科学基金(2020GXNSFAA238021,2021GXNSFDA075008);北京市高层次公共卫生技术人才建设项目(2020-2-024)
作者单位
产柳佳 南宁广西中医药大学药学院2020级研究生2班 
杨舒雯 首都医科大学第六临床医学院2018级五年制临床医学3班 
闫亚冬 首都医科大学附属北京佑安医院 北京市肝病研究所 
王文敬 首都医科大学附属北京佑安医院 北京市肝病研究所 
李?榕 首都医科大学附属北京佑安医院 北京市肝病研究所 
陈?静 北京市中西医结合传染病研究所 
李伟华 北京市中西医结合传染病研究所 
赵立春 广西药食同源资源开发重点实验室 
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中文摘要:
      [摘要]?目的?探讨黄芪多糖对肝癌Hep G2.215细胞体外增殖的抑制作用及其作用机制。方法?CCK-8法测定不同浓度黄芪多糖作用于Hep G2.215细胞48 h、72 h后对其增殖影响;平板细胞克隆实验测定不同浓度黄芪多糖对Hep G2.2.15细胞克隆生长的影响;流式细胞术检测黄芪多糖对Hep G2.215细胞周期和凋亡的影响。结果?CCK-8检测结果显示,当黄芪多糖给药浓度达1000 μg/ml时,对Hep G2.215细胞生长具有抑制作用,且与对照组相比差异有统计学意义(P<0.05)。平板细胞克隆实验表明,低浓度(100 μg/ml)的黄芪多糖对Hep G2.215细胞克隆形成具有抑制作用,与对照组比较,差异有统计学意义(P<0.05)。流式细胞术检测结果显示,G2/M期细胞比例与对照组相比呈下降趋势(P<0.05),经过黄芪多糖处理的Hep G2.215细胞,细胞凋亡率高于对照组(P<0.05)。结论?黄芪多糖对肝癌Hep G2.215细胞生长具有抑制作用,其机制可能是通过阻滞Hep G2.215细胞进入G2/M期,激活了细胞凋亡系统,从而诱导细胞凋亡。
英文摘要:
      [Abstract]?Objective? Investigate the inhibitory effects of Astragalus polysaccharides on the proliferation of HBV positive hepatocellular carcinoma cells Hep G2.215 in vitro and the mechanism of this process. Methods?CCK-8 assay was used to determine the effect of different concentrations of Astragalus polysaccharides on the proliferation of Hep G2.215 cells for 48 h and 72 h. The effects of different concentrations of Astragalus polysaccharides on the clonal growth of Hep G2.2.15 cells were determined by plate cell cloning assay. Flow cytometry was used to detect the effects of astragalus polysaccharide on the cell cycle and apoptosis of Hep G2.215. Results?The CCK-8 assay experiments showed that when Astragalus polysaccharides concentration was 1000 μg/ ml, it had an inhibitory effect on the growth of Hep G2.215 cells, and the difference was statistically significant compared with the control group (P<0.05). The plat cell cloning experiments showed that a low concentration of Astragalus polysaccharides (100 μg/ ml) could inhibit the cloning of Hep G2.215 cells, and the difference was statistically significant compared with the control group (P<0.05). Flow Cytometry results showed that the proportion of cells in the G2/M phase decreased compared with the control group (P<0.05, and the apoptosis rate of Hep G2.215 cells treated by Astragalus polysaccharides was higher than that of the control group (P<0.05). Conclusions?The Astragalus polysaccharides can inhibit the growth of hepatocellular carcinoma Hep G2.215 cells, and the mechanism may be that Astragalus polysaccharides block Hep G2.215 cells from entering the G2/M phase, activate the apoptosis system and induce apoptosis. ???ā??Lucida Sans Unicode?Ё蠤?
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