| [Abstract]?Objective?To investigate and analyze the spatial and temporal distribution characteristics of tuberculosis incidence and its gender and age in Nanjing. Methods?Pulmonary tuberculosis patients registered in designated hospitals in Nanjing from 2016—2020 were selected as the study subjects. Annual trends in incidence were assessed by estimated annual percent change (EAPC). Regional reported incidence was standardized according to the age of Nanjing population in the sixth national census in 2010. Results?From 2016—2020, there were 11 406 tuberculosis patients in Nanjing, namely 5023 patients (44.04%) with positive etiology, 5812 patients (50.96%) with negative etiology, 285 patients (2.50%) with resistant to rifampicin, and 285 patients (2.50%) without etiology results. The EAPC for tuberculosis incidence was -2.08%, which means the overall reported incidence of tuberculosis decreased, but the annual reported incidence of tuberculosis was not statistically significant from 2016—2020 (P＞0.05). And there was no significant difference between gender of tuberculosis patients (P＞0.05). However, when patients were stratified by age, we found that the lowest incidence in patients ＜15 years old (0.07/100 000～0.19/100 000)and the highest incidence in patients 25 to 34 years old (5.14/100 000～5.71/100 000)The standardized incidence of tuberculosis in each region was 18.80/100 000～39.30/100 000. The top 3 areas were Gaochun District (39.30/100 000), Jiangning District (35.50/100 000) and Jiangbei New Area (33.10/100 000). The areas with low standard incidence were Qinhuai District(18.80/100 000)、Gulou District(19.80/100 000)and Xuanwu District(19.80/100 000); and xuanwu District (19.80/100 000), the Moran index I were 0.256, 0.370, 0.370, 0.370, 0.352 and 0.305 respectively, and the differences were significant (P＜0.05); local spatial autocorrelation analysis showed that 2, 1, 1, 3, and 1 districts (countries) were in HH from 2016—2020. Lishui District was in the HH area for 5 consecutive years. Pukou District was in HH area in 2016、2019 respectively, Jianye District and Qinhuai District were in LL area in 2017—2019, Qixia District in HH area in 2019, and Gulou District in LL area in 2019. Conclusions?From 2016—2020, the incidence of tuberculosis in Nanjing showed a decline, and incidence reported in each year showed a significant clustered distribution, and had great differences in different spatial, temporal and age distribution. Therefore, we should take effective prevention and control measures by combining its epidemiological characteristics.