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| 化学发光法与胶体金法检测SARS-CoV-2抗体阳性率比较 |
| Comparison of chemiluminescence method and colloidal gold method for detecting SARS-CoV-2 antibody positive rate |
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| DOI：10.3969/j.issn.1007-8134.2020.06.010 |
| 中文关键词: 新型冠状病毒 化学发光法 胶体金法 抗体 |
| 英文关键词: SARS-CoV-2 chemiluminescence method colloidal gold method antibody |
| 基金项目:宁夏医科大学总医院新冠肺炎疫情防控应急项目（XE202010，XE202018）；宁夏医科大学校级科研项目（XY2017132）；宁夏青年科技人才托举工程项目（TJGC20190096，TJGC20190099） |
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| 中文摘要: |
| 目的 探讨化学发光法和胶体金法检测SARS-CoV-2抗体在2019冠状病毒病（coronavirus disease 2019, COVID-19）诊断中的应用价值。方法 收集51例COVID-19确诊患者和41例非COVID-19患者的血清，用化学发光法检测非COVID-19组血清中SARS-CoV-2抗体IgM、IgG及联合检测（IgM或IgG）的阳性率；用化学发光法和胶体金法检测COVID-19组血清中SARS-CoV-2抗体IgM、IgG及联合检测（IgM或IgG）的阳性率，比较2种方法的一致性。结果 COVID-19组平均发病时间为（16.9±5.4）d。其中22例IgM阳性，阳性率为43.1%；47例IgG阳性，阳性率为92.2%；49例联合检测（IgM或IgG）阳性，阳性率为96.1%。非COVID-19组中，1例IgM阳性，阳性率为2.4%；0例IgG阳性，阳性率为0；1例联合检测（IgM或IgG）阳性，阳性率为2.4%。在COVID-19组中，化学发光法与胶体金法检测SARS-CoV-2抗体联合检测（IgM或IgG）的阳性率分别为96.1%和68.6%，化学发光法优于胶体金法（P＜0.05）。结论 SARS-CoV-2抗体的检测对COVID-19诊断有重要的意义，且化学发光法检出率优于胶体金法。 |
| 英文摘要: |
| Objective To explore the application of SARS-CoV-2 antibodies in diagnosis of coronavirus disease 2019 (COVID-19) by chemiluminescence method and colloidal gold method. Methods The sera were selected from 51 patients with confirmed COVID-19 and 41 patients without COVID-19. The positive rates of antibody IgM, IgG and combined detection (IgM or IgG) in serum of non-COVID-19 group were detected by chemiluminescence method, and the positive rate of antibody IgM, IgG and combined detection (IgM or IgG) in patients of COVID-19 group was detected by chemiluminescence method and colloidal gold method, then the consistency of 2 methods was compared. Results In the COVID-19 group, the average onset time of patients was (16.9±5.4) days. There were 22 cases positive for IgM, 47 cases positive for IgG and 49 cases positive for combined dection, with the positive rates of 43.1%, 92.2% and 96.1%. In the non-COVID-19 group, there was 1 case positive for IgM, 0 case positive for IgG and 3 cases positive for combined dection, with the positive rate of 2.4%, 0 and 2.4%. Among the 51 COVID-19 cases, the positive rates of SARS-CoV-2 antibody combined dection by the chemiluminescence method and colloidal gold method were 96.1% and 68.6%. The chemiluminescence method was better than the colloidal gold method (P＜0.05). Conclusions The detection of antibodies against SARS-CoV-2 is of great significance for the diagnosis of COVID-19, and the detection rate of chemiluminescence method is superior to that of colloidal gold method. |
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