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| 互作转录组测序技术在医学中的应用 |
| Application of Dual RNA-seq in medicine |
| 投稿时间：2023-12-27 修订日期：2024-04-24 |
| DOI： |
| 中文关键词: 互作转录组测序 感染 生物信息学 |
| 英文关键词: Dual RNA-seq Infect bioinformatics |
| 基金项目:国家重点研发计划资助项目 (2020YFC2002706-2) |
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| 摘要点击次数: 27 |
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| 中文摘要: |
| 【 】 伴随着第二代、第三代测序技术的发展,转录组测序技术的测序深度不断增加,并且在现今的临床医学诊断和治疗中的应用越来越广。互作转录组测序技术(Dual RNA-seq),可以同时提取两个(或多个)互作物种的RNA,反映它们相互作用下的基因表达情况,在医学上可用于分析感染患者或实验样本和病原体之间转录组水平的互作情况,从而评估患者或实验样本基因表达状态并用于药物预测。本文主要综述了Dual RNA-seq的工作原理及其在医学上的应用,并介绍了其与单细胞测序和非编码RNA分析结合的进展,以期对感染相关的临床和研究工作提供参考。 |
| 英文摘要: |
| 【 】 With the development of Next-generation sequencing and The Third-generation sequencing technology, the sequencing depth of transcriptome sequencing technology is increasing, and the application in today"s clinical diagnosis and treatment is becoming more and more extensive. Dual RNA-seq can extract the RNA of two (or more) interacting species at the same time, reflecting the gene expression under the interaction of them, and can be used in medicine to analyze the interaction of the transcriptome between infected patients or experimental samples and pathogens. This allows to assess the gene expression status of patient or experimental samples and use them for drug prediction. This review summarizes the working principle of Dual RNA-seq and its application in medicine, and introduces its progress in combination with single-cell sequencing and non-coding RNA analysis, in order to provide some references for clinical and research work related to infections. |
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